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. 1 Globodera rostochiensis () G. rostochiensis. . TG-689141, 1. . . 1 70 , 67 (95,7%) , , 3 -- . G. rostochiensis . 60 . 10 , . . 60 (85,7%) . ; ; ; Globodera rostochiensis (Woll.) () , . - Solanum tuberosum L. Solanum. , . , , . - . , [1, 2]. 30%. , [1, 2].
Solanum tuberosum subsp. andigenum (Juz. & Bukasov) 1, , , 䳿. 1, V , Ro1 Ro4 [3].
- , , . - . H1 - () [3_6]. TG-6891/41.
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. NeoPrep_100 (, ).
TG689141, H1, TG689-allele-specific (55-TAAAACTCTTGGTTATAGCCTAT-35) TG689-indel (55-CAATAG AATGTGTTGTTTCACCAA-35) [7]. , , 141 .. -- BCH-F2 (55-CGTTTGGCGCTGCCGTAAGTT-35) BCH-R2 (5'-CATGACATAGTTTGAATTTTGAGTC-3')C 290 .. [7].
PCR MIX 2x HOT (, ). Applied Biosystems 2720 Thermal Cycler. - TG-689141 BCH: -- 12 94C, 35 ( 20 94C, 20 55C, 30 72C), -- 7 .
2% 1 . - VISION Gel.
[1]. 60- . : 1 () -- 0 ; 2 () -- 1--5 ; 3 () -- 5 .
ϳ - , ( ). - , 2- , () .
. TG689141 1, H1 141 ..
. 1. TG689141 H1 1, 3, 4^, 8, 11, 13--17; M -- 100 bp DNA Ladder
1 (1 ) TG689141. 1 70 , 67 , ' , 3 -- . 60 . 10 , . 7- , , , , , . 85,7% .
H1, - G. rostochiensis, V . G. rostochiensis. Galek et. al. , [7]. 50% , [7].
. 20 20 , (74% 90% ) [8]. . ³ , . , , , .
, . TG 689141 -- 92% [9]. 32% 95,6% (. 2).
2. 1 (G. rostochiensis) TG689141
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Galek et. al. [7] |
94 |
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. [8] |
74 -- 90 -- |
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, . [9] |
92 |
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Milczarek et. al. [10] |
95,6 |
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. [11] |
87,6 |
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.[12] |
32,4 |
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85,7 |
1 67- (95,7%). , . ³ ( ) 85,7%.
Գ: 24. Գ- , ( ) ϳ 01. (Գ) 24.01.02.02 - 볻 0121U000093.
: .
˲Բ
1. ѳ .., .., .., .. ѳ . : , 2017. 340 .
2. ϳ .., .., .. . . ѳ . 2020. 16. . 149-159. DOI: 10.37128/2707-5826-2020-1-11
3. Bhardwaj Umamaheshwari R., Sharma R., et.al. Potato cyst nematode (PCN) resistance: genes, genotypes and markets-an update. SABRAO Journal of Breeding and Genetics. 2012. 44. . 202-208.
4. Niewohner J., Salamini F. Gebhardt C. Development of PCR assays diagnostic for RFLP marker alleles closely linked to alleles Grol and H1, conferring resistance to the root cyst nematode Globodera rostochiensis in potato. Mol. Breeding. 1995. 1, 65-78. doi: 10.1007/BF01682090
5. Milczarek, D., Flis, B. Przetakiewicz, A. Suitability of Molecular Markers for Selection of Potatoes Resistant to Globodera spp. Am. J. Pot Res. 2011. 88. 245-255. doi: 10.1007/s12230-011-9189-0
6. Asano K., Kobayashi A., Tsuda S., Nishinaka M., Tamiya S. DNA marker- assisted evaluation of potato genotypes for potential resistance to potato cyst nematode pathotypes not yet invading into Japan. Breed Sci. 2012. 62(2), 142-50. doi: 10.1270/jsbbs.62.142
7. Galek R., Michal R., Walter S. De Jong J. Application of DNA markers linked to the potato H1 gene conferring resistance to pathotype Rol of Globodera rostochiensis. Appl. Genet. 2011. 52, P. 407-411. doi: 10.1007/s13353-011-0056-y
8. Karelov A.V., Pylypenko L.A., Kozub N.O., Sozinov I.O. et al. (2013). Allelic state of the molecular marker for golden nematode (Globodera rostochiensis) resistance gene H1 among Ukrainian and world potato (Solanum tuberosum ssp. tuberosum) cultivars. Cytol. Genet. 47, 294-297. doi: 10.3103/S0095452713050058
9. .., .. - . - . 2018. . 32. 12. . 47-49.
10. Milczarek D., Przetakiewicz A., Kaminski P., Flis B. Early selection of potato clones with the H1 resistance gene -- the relation of nematode resistance to quality characteristics. Czech J. Genet. Plant Breed. 50(4), 278-284. doi: 10.17221/114/2014-CJGPB
11. Antonova O.Yu., ShvachkoN.A., NovikovaL.Yu., etall. Genetic diversity of potato varieties bred in Russia and near-abroad countries based on polymorphism of SSR-loci and markers associated with resistance R-genes. Vavilovskii Zhurnal Genetiki i Selektsii, Vavilov Journal of Genetics and Breeding. 20(5), 596-606. doi: 10.18699/VJ16.181
12. .., .., .. . . _ . 2018. 22(1). . 18-24.
Kyrychenko S., Sozinova O., Bondar T.,
Sozinov I., Kozub N., Borzykh O.,
Institute of Plant Protection of NAAS, Kyiv
Analysis of Ukrainian potato breeding lines using the DNA marker for the H1 potato cyst nematode gene GoA, Molecular identification of carriers of the H1 gene among potato varieties of the Polish research department of the Institute of Potatoes of the National Academy of Sciences (IC) and comparison of these data with the results of the biotest analysis for resistance to Globode^a ros^ochiensis. Methods, The use of molecular markers that indicate the detection of the resistance allele in the genomic of the variety. The PCR method was used. The lines were also analyzed by a biotest method. Resets, Screening of allele of the molecular marker H1 gene in a selection of 70 cultivar samples showed that 67 (95.7%% samples have the allele associated with resistance, and 3 _ the allele for susceptibility. The paper presents the results of the study of the resistance of potato breeding lines of the Ukrainian breeding of 1--2 years of testing, provided by the Polish Research Station. 60 resistant variety samples were established by the biotest method. 10 variety samples were noted, in which the results of analysis by the molecular methods and bioassay did not match. Conclusions. Therefore, a comparison of the bioassay method and molecular studies showed a coincidence of results in 60 (85.7%c) resistant breeding lines in the study.
Golden potato cyst nematode; resistance genes; molecular markers; potato
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