Phosphorous balance in different fertilization systems in agrocenosis of the Central Forest-Steppe
Comparative assessment of phosphorus balance in agrocenosis. Establishing a connection with the yield of grain crops in a short crop rotation with long-term use of organic and organo-mineral fertilizer systems in the Central Forest-Steppe of Ukraine.
Рубрика | Сельское, лесное хозяйство и землепользование |
Вид | статья |
Язык | английский |
Дата добавления | 12.08.2022 |
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Phosphorous balance in different fertilization systems in agrocenosis of the Central Forest-Steppe
Demydenko O., Cherkasy State Agricultural Research
Station of National Institute of Agriculture of NAAS
Goal. To give a comparative assessment of the balance of phosphorus, and to determine a connection with the productivity of cereals in the short crop rotation for long-term application of organic and organo- mineral fertilizer systems in the Central Forest-Steppe of Ukraine. By-products of crops were used as organic fertilizer at saturation of the crop rotation with the legume. Methods. Studies were carried out in the stationary field experiment in the Cherkasy state agricultural experimental station of NSC «Institute of agriculture of NAAS» in 2011 - 2019 on the area of 0.75 ha in 5 fields with the seed size of the plot 30 m2, and 4-times repeatability. They used field, laboratory, comparative-calculation, and mathematical methods. Results. At the use of organic fertilizer system, a strong correlation was revealed between the output of f.u. and the main products and the rate of turnover and balance capacity of P2O5: in the first case, the opposite (R=0.65-0.69±0.02; R2=0.42-0.48), in the second -- direct (R=0.67- 0.85±0.02; R2=0.45-0.72). Compared to the organo-mineral fertilizer system correlation weakened somewhat. Per unit of output of f.u. and the primary product increase of the capacity of the P2O5 balance was in 1.78 and 2.11 times smaller. Between the P2O5 balance capacity and balance capacity of N and CO2 was a direct correlation on the average level: R=0.53±0.02; R2=0.23 for N, and R=0,90±0,03; R2=0,86 -- for CO2. Per unit of the capacity growth of balance of phosphorus, they fixed 7.62 kg N and 0.79 tons of CO2, which was significantly higher as compared to the organo-mineral fertilizer system. Conclusions. Assessment of dynamics of yield of grain crops in 2011 - 2019 had shown the following: in organic fertilizer system the increasing trends were detected in winter wheat and barley; in organo-mineral fertilizer system regression coefficients for the variable degree functions at the cultivation of barley were higher in 1.5-1.8 times, winter wheat -- were approaching each other. Grain yields at the use of organic and organo-mineral fertilizer systems of cultivation were decreasing, at the use of organic fertilizer system, the drop in trend was in 1.9 times less.
Key words: organic fertilizer system, organo-mineral fertilizer system, turnover of phosphorus, intensity of balance, capacity of balance, winter wheat, spring barley, corn.
Introduction
The phosphorus cycle in agrocenoses under different fertilization systems and different levels of intensity has a certain direction, dynamism, as well as stability and its specific state. It can be quantified using the balance of phosphorus - a kind of identification system, which indicates the existing features and measures required for stabilization and normal functioning of the phosphorus cycle under different fertilizer systems [13]. The priority macronutrients, the balance of which must be determined, include, at the level of organic carbon and nitrogen, phosphorus [4, 5]. Phosphorus is one of the key elements of mineral nutrition of crops, which plays an important and central role in their metabolism [6-10]. Creating an energy basis for the functioning of plant cells, it is part of nucleic acids and nucleotides, membrane lipids, enzymes and intermediates of the photosynthetic and respiratory cycles, and therefore its assimilation and metabolism are crucial for plant growth and development. Although the initial stages are critical for phosphorus, optimal supply of them is important throughout the growing season of plants under different fertilizer systems.
Phosphorus by its chemical properties has a complex nature of interaction with soil components, which greatly complicates the objective assessment of soil availability of this element for optimal growth and development of plants under different fertilization systems, and the availability of phosphates is determined by the solubility of fertilizer reaction products. It is very important to maintain the level of phosphorus available to plants to avoid its deficiency, which in turn leads to a decrease in crop yields [11-13]. Under the influence of intensification of agricultural production, growth of crop yields and intensification of soil degradation processes that took place in the first decades of the 21st century, other agro-ecological conditions have been formed that need new criteria for evaluation and revision of existing ones. Many scientific works have been devoted to the study of the influence of fertilizer systems on the phosphorus balance in crop rotation agrocenoses [14-21]. and soy. Topicality. At the present stage of formation of organic farming the question of formation of balance of phosphorus and its influence on productivity of cultures becomes short.
Balance calculations of phosphorus were performed according to an improved method [22] of calculating the balance of nutrients in on-farm land management projects taking into account the removal of phosphorus by the main crop, and phosphorus circulation time is calculated as the ratio of phosphorus in total phytomass to vegetation consumption [22]. Generalization of research results was performed using the program STATISTICA-10 using non-parametric statistics and correlation analysis.
1. Organic fertilizer system in 5-row grain-row crop rotation
Crop rotation crops |
NRC income, kg / ha: |
||||||||||
inoculati on |
with byproducts |
on straw for fertilizer |
feeding with biological product |
||||||||
NPK |
N |
Р |
К |
N |
Р |
К |
N |
Р |
К |
||
pea |
50/50/50 |
22 |
8 |
47 |
87 |
57 |
18 |
50 |
50 |
50 |
|
Winter wheat |
50/50/50 |
31 |
7 |
42 |
44 |
68 |
38 |
50 |
50 |
50 |
|
Corn |
50/50/50 |
45 |
14 |
92 |
25 |
46 |
22 |
50 |
50 |
50 |
|
Soy |
50/50/50 |
33 |
10 |
64 |
33 |
85 |
41 |
50 |
50 |
50 |
|
Barley |
50/50/50 |
27 |
3 |
31 |
7 |
42 |
19 |
50 |
50 |
50 |
* Standard consumption of mineral fertilizers for the formation of 1 ton of crop, kg/ha
Research results
phosphorus balance fertilizer
The general statistical model of phosphorus content (P2O5) in the structure of total phytomass for organo-mineral and organic fertilizer systems showed that the interval content is Amax-min = 20.7 kg/ha with an average content of 33.4 kg/ha. The median value of P2O5 content is close to the average (34.2 kg/ha), and the typical range of values is AL0.75-L0.25 = 7.6 kg/ha. The phosphorus content in the total phytomass averages 46.8 kg/ha with an interval scale of 20.1 kg/ha. The content of P2O5 at the median value deviated slightly from the average value (48.1 kg/ha), and the typical range of values was L0.75-0.25 = 6.5 kg/ha.
The content of P2O5 at the median value tends to a greater extent to the upper typical value, which indicates a tendency to increase P2O5 in the total phytomass. The content of P2O5 of the main product in the total phytomass by average and median values is 71-72%. 28-29% of P2O5 from the total content in phytomass is concentrated in by-products and roots: the interval range is Amax-min = 6.5 kg/ha, and the typical interval is AL0.75-0.25 = 3.7 kg/ha. The coefficients of variation of the structuring parameters of the total phytomass varied within the allowable limits of Coef * v = 21.9%.
The use of organo-mineral fertilizer system promotes the growth of both average and median P2O5 content in the main product, which increases by 9-10% or 3.4-3.9 kg/ha, respectively, the general model. The interval scope of the content is narrowed due to the increase of the minimum value of the content by 9.4 kg/ha. The typical content range for the organo-mineral fertilizer system narrows due to the increase in the values of the upper (L0.75) and lower (L0.25) typical values by 3.2-5.4 kg/ha. The average weight of the total phytomass relative to the values of the general model increased by 5.9 kg/ha, and the median weight - by 6.4 kg/ha. The interval and typed scope narrowed relative to the overall model by 1.24 and 1.39 times. The coefficient of variation of the parameters of the components of the total phytomass was Coef * v = 10.310.9% (table 2).
2. Statistical parameters of phosphorus content in the structure of phytomass of short-rotation crop rotation under different fertilizer systems (2010-2019)
Statistical parameters |
Р2О5 в (t/ha): |
|||||
main products |
by-products |
roots |
phytomass |
|||
Organo-mineral fertilizer system (control) |
||||||
****Parameter |
average |
37,1 |
15,1 |
0,26 |
52,7 |
|
min |
29,6 |
11,6 |
0,21 |
41,5 |
||
max |
40,9 |
17,0 |
0,29 |
57,7 |
||
*** Quantum parameter |
Med, 0.50 |
38,1 |
15,9 |
0,28 |
54,5 |
|
L 0.25 |
34,9 |
14,1 |
0,25 |
49,4 |
||
L 0.75 |
40,3 |
16,4 |
0,28 |
57,3 |
||
Coef. V, % |
10,6 |
12,0 |
10,3 |
10,9 |
||
Organic fertilizer system |
||||||
****Parameter |
average |
30,5 |
13,0 |
0,22 |
43,9 |
|
min |
25,2 |
11,7 |
0,19 |
40,2 |
||
max |
35,3 |
16,1 |
0,25 |
50,9 |
||
*** Quantum parameter |
Med, 0.50 |
30,1 |
12,2 |
0,21 |
42,5 |
|
L 0.25 |
28,1 |
11,8 |
0,20 |
40,9 |
||
L 0.75 |
33,4 |
14,0 |
0,23 |
46,7 |
||
*Coef. V, % |
11,7 |
12,5 |
9,63 |
8,97 |
Note: * Coef. V,% - coefficient of variation; *** max-min - amplitude range; **** L 0.75- L 0.25 - quartile scope or normalized scope.
Under the organic fertilizer system, the average and median stock of P2O5 in the main products relative to the intensive fertilizer system decreased by 1.22-1.27 times. The interval scope of the P2O5 stock was at the level of the intensive fertilizer system at lower values of the minimum and maximum stock values: 28.1-33.4 kg/ha against 29.6-40.9 kg/ha. Similarly, a typical range of P2O5 stock in the main products was formed: the upper and lower typical values were lower by 1.05 and 1.22 times the value of the organo-mineral fertilizer system. If in the organo-mineral system of fertilizers the median value tended to the upper typical value, in the organic, on the contrary - to the lower typical value, which indicates the restraint of the accumulation of P2O5 in the main products (Table 2).
3. Statistical parameters of the phosphorus balance of short-rotation crop rotation under different fertilizer systems for 2010-2019
Statistical parameters |
Вalance P2O5 (kg) |
Balance (kg/ha) |
The intensity of the balance of P2O5,% |
Turning speed, P2O5 |
Balance capacity, P2O5, kg/ ha |
||
Organo-mineral fertilizer system (control) |
|||||||
****Parameter |
average |
+17,7 |
+3,54 |
124 |
0,71 |
164 |
|
min |
+19,4 |
+3,88 |
129 |
0,62 |
153 |
||
max |
+14,1 |
+2,82 |
118 |
0,75 |
169 |
||
*** Quantum parameter |
Med 0.50 |
+17,4 |
+3,48 |
123 |
0,73 |
165 |
|
L 0.25 |
+19,1 |
+3,82 |
127 |
0,69 |
159 |
||
L 0.75 |
+14,9 |
+2,98 |
114 |
0,74 |
168 |
||
Coef. V, % |
13,4 |
13,4 |
3,82 |
6,16 |
3,82 |
||
Organic fertilizer system |
|||||||
****Parameter |
average |
-16,6 |
-3,32 |
46,0 |
1,44 |
44,4 |
|
min |
-11,0 |
-2,20 |
56,0 |
1,39 |
39,0 |
||
max |
-19,0 |
-3,80 |
47,0 |
1,45 |
53,0 |
||
*** Quantum parameter |
Med 0.50 |
-18,0 |
-3,60 |
42,0 |
1,42 |
43,0 |
|
L 0.25 |
-16,0 |
-3,20 |
44,0 |
1,40 |
41,0 |
||
L 0.75 |
-18,2 |
-3,64 |
45,2 |
1,43 |
48,0 |
||
Coef. V, % |
23,7 |
23,0 |
18,8 |
5,9 |
8,6 |
Note: * Coef. V,% - coefficient of variation; *** max-min - amplitude range; **** L 0.75- L 0.25 - quartile scope or normalized scope.
The average median stock of P2O5 in the total phytomass of the organic fertilizer system was lower by 1.20 and 1.35 times relative to the organo-mineral fertilizer system. The interval and typical range of P2O5 stock was narrower relative to the organo-mineral fertilizer system, and Coef * v were in the range of 8.9712.5%, which indicates the stabilization of the process of P2O5 accumulation in the structural elements of phytomass. The main products accounted for 69-70% of P2O5 from the accumulated stock in the total phytomass. According to the organo-mineral fertilizer system, the average phosphorus yield for all articles was 90.5 kg/ha with an amplitude range Amax-min = 5.6 kg/ha. The median yield was at the level of the average value (91.0 kg/ha), and the typical interval range was AL0.75-0.25 = 2.4 kg/ha, which indicates a stabilization in time of P2O5 entry into the balance circulation of phosphorus in crop rotation.
The income of P2O5 due to the lateral, aboveground and root phytomass was 17-18% of the total income of both average and median values and amounted to 15.6-16.3 kg/ha. The value of the receipt of P2O5 for this article by the median tended to the upper typical value, which indicates a growing trend of a positive article P2O5.
The balance of P2O5 on the average and median values was positive at the level of + 17.4-17.5 kg/ha. The interval and typical scope were narrowed, which indicates the stability of the P2O5 balance in crop rotation under the organo-mineral fertilizer system. The balance capacity of the organo-mineral fertilizer system was 164-165 kg/ha, and the interval amplitude and normalized range of values was Amax-min = 16.0 kg/ha and AL0.75-0.25 = 9.0 kg/ha. The coefficient of variation was at the level of Coef * v = 3.82%, which indicates the stability of the balance capacity over the years of research. The turnover rate of P2O5, which was determined by the ratio of P2O5 content in the total biomass to the consumption of P2O5 during the growing season (B), was <1.0, which indicates a high level of circulation. Amplitude and normalized interval values of P2O5 turnover rate were Amax-min = 0.13 kg/ha and AL0.75-0.25 = 0.05 kg/ha, which indicates the intensive circulation of P2O5 at low values of Coef * v <10,0. The values of the balance intensity (IBsr = 0.5) significantly exceeded 100%, which confirms the conclusion about the high level of P2O5 circulation in the agrocenosis of short-rotation crop rotation during 8 years of research (Table 3).
Under the organic fertilization system, the yield of P2O5 on all balance sheet items was 6.5 times lower than the average value and 7.2 times lower than the median value. The amplitude range was 4.4 kg/ha at absolute values of deviation of 7.2-7.3 times less compared to the control variant. Receipt of P2O5 was provided by the content of P2O5 in by-products. The removal of P2O5 by the main products was 2.39-2.45 times lower. The limits of the interval range and the limits of the typical values of P2O5 removal were 2.162.67 and 2.30-2.45 times smaller compared to the organo-mineral fertilizer system, which affected the level of P2O5 balance, which was negative and was characterized by the intensity of the balance Ib <100% and did not exceed 50%. The balance capacity under the organic fertilizer system was 3.46-3.82 times lower, as well as the interval amplitude and normalized typical values. The turnover rate of P2O5 was 1.94-2.05 times lower, and the value of B was higher than one: B>1.33-1.45, which indicates a low intensity of circulation of P2O5 in the agrocenosis of short-rotation crop rotation under the organic fertilizer system (table 3).
The evaluation of the balance of different models of fertilizer systems in short-rotation crop rotation was performed on the set of paired correlations between the set of features in the matrix correlation field. In the general model of the correlation matrix of paired correlation coefficients (n=54), direct and inverse correlations (R>0.50) account for 39% of interactions. Of these, 39% are direct strong correlations, 27.8% are inverse strong correlations, and the ratio between them is 2.5 to 1 in favor of functional (direct) connections, and the connections that stabilize the system (inverse) account for 11, 1%. There were 61% of the total number of functionally insignificant connections (± R <0.50).
In the organo-mineral system, the fertilization of strong bonds (± R> 0.5) was 44%. Of these, 37% - functional connections, 7.4% - stabilizing, inverse, and the ratio of functional and stabilizing connections was 5 to 1. Relationships insignificant (± R <0.5) were 55.6%. In the conditions of the organic system, the fertilization of functional bonds as a whole was 52%; direct action - 35.2%; reverse action - 16.7% at a ratio of 2.1 to 1. Non-essential connections as in the previous case was 48.2%.
According to the fertilizer model, the yield of k.o. and the balance capacity P2O5 correlate at the level of direct strong correlation (R = 0.87-0.96 ± 0.02; R2 = 0.76-0.92), and per unit of output growth k. and the main products account for an increase in the capacity of the balance of P2O5 7.63-10.7 kg. In this case, the speed of rotation P2O5 and crop rotation productivity at the output of k.o. and the main product correlates with each other at the level of weak correlation.
It is established that a direct correlation is established between the P2O5 balance capacity and the output of the main product in 5-field grain-row crop rotation according to the general model at the level of strong correlation, and the unit of phosphorus balance capacity growth is 0.008 t/ha of the main product yield.
4. The regression equation between the capacity of the balance of phosphorus, nitrogen and the yield of the main product in the grain-row 5-row crop rotation under different fertilizer systems
Correlation parameters |
Regression equation |
Correl ation coefficient |
|
General model |
|||
Balance capacity-P2O5, kg: main products, t/ha |
y = 3,64 + 0,008*x |
0,65 |
|
Balance capacity-P2O5: balance capacity N, kg/ha |
y = 524,2 + 1,14*x |
0,67 |
|
Organo-mineral fertilizer system (control) |
|||
Balance capacity-P2O5, kg: main products, t/ha |
y = -8,76 + 0,08*x |
0,95 |
|
Balance capacity-P2O5: balance capacity N, kg/ha |
y =-275,2 + 6,03*x |
0,65 |
|
Organic fertilizer system |
|||
Balance capacity-P2O5, kg: main products, t/ha |
y = 0,87 + 0,07*x |
0,65 |
|
Balance capacity-P2O5: balance capacity N, kg/ha |
y = 242,9 + 7,62*x |
0,49 |
The capacity of the phosphorus balance determines the yield of the main product by 64%. There is a direct correlation between the capacity of the phosphorus and nitrogen balance at the level of strong correlation, and the unit of growth of the phosphorus balance is the increase of the nitrogen balance capacity by 1.15 units (Table 4).
The organo-mineral fertilizer system also shows a direct correlation between the phosphorus balance capacity and the yield of the main product at the level of strong correlation, and 0.08 units of the output of the main product per unit growth of the balance capacity. The capacity of the phosphorus balance determines the yield of the main product by 90%. A direct strong correlation was found between the phosphorus-nitrogen balance capacity, and 6.03 units of nitrogen per unit of phosphorus growth capacity growth (Table 3).
Fig. 1. Dynamics of yield of winter wheat and spring barley depending on the fertilizer in the grain- row 5-field crop rotation for 2011-2019
Fig. 2. Dynamics of corn yield per grain depending on the fertilizer in the grain-row 5-field crop rotation for 2011-2019
Under the organic fertilization system, the relationship between the capacity of the phosphorus balance and the yield of the main product was at the level of direct strong correlation, and the unit of growth of the capacity of the phosphorus balance is 0.07 units of growth of the main product. The capacity of the phosphorus balance determines the yield of the main product by 90%. The capacity of the phosphorus balance determines the yield of the main product by 60%, which is 1.5 times less efficient compared to an intensive fertilizer system. A direct correlation at the level of the average correlation was found between the phosphorus and nitrogen balance capacities. Phosphorus balance capacity determines an increase in nitrogen balance capacity of 24%, which is 1.3 times less efficient compared to an intensive fertilizer system.
5. Grain yield parameters for different fertilizer systems (2011-2019)
Yield, t / ha |
Typical yield, t / ha |
*C oef V, % |
||||||
Fertilizer system |
min |
max |
med |
L 0.25 |
L 0.75 |
|||
**max-min |
P 0.50 |
***i і L 0.75- L 0.25 |
||||||
Winter wheat |
||||||||
Organo-mineral |
6,24 |
4,21 |
7,24 |
6,78 |
5,44 |
7,00 |
17, 5 |
|
Organic |
5,16 |
3,55 |
6,75 |
5,10 |
4,65 |
5,75 |
18, 7 |
|
Maize |
||||||||
Organo-mineral |
9,99 |
5,84 |
13,2 |
10,1 |
8,84 |
11,50 |
22, 4 |
|
Organic |
8,10 |
5,51 |
10,4 |
7,96 |
7,50 |
8,98 |
18, 2 |
|
Spring barley |
||||||||
Organo-mineral |
3,63 |
2,55 |
4,49 |
3,74 |
3,10 |
4,18 |
20, 3 |
|
Organic |
2,85 |
1,45 |
3,81 |
3,00 |
2,08 |
3,52 |
32, 5 |
|
Cereals |
||||||||
Organo-mineral |
6,67 |
5,18 |
7,50 |
6,78 |
6,46 |
7,10 |
10, 5 |
|
Organic |
5,35 |
4,69 |
5,92 |
5,37 |
5,05 |
5,70 |
7,9 5 |
Note: * Coef. V,% - coefficient of variation; *** max-min - amplitude range; **** L 0.75- L 0.25 - quartile scope or normalized scope.
Assessment of the dynamics of grain yields for 2011-2019 under the organic fertilizer system showed that increasing trends were found in winter wheat and barley. Compared with the growth trends of yields of these crops in the organo-mineral system of cultivation, in the organic system the regression coefficients with variable power function for growing barley were 1.5-1.8 times higher, and for growing winter wheat approached each other. When growing corn in the organic system, the trend of yield change was downward, as in the organo-mineral fertilizer system, but in the organic fertilizer system, the yield trend was less downward. In general, grain yields in both organic and organo-mineral cultivation systems were declining, but in the organic fertilization system the decline of the trend was 1.9 times less compared to the intensive fertilization system (Fig. 1, 2).
Statistical evaluation of yield for 2011-2019 showed that the average yield of winter wheat, corn, barley was at the level of 5.16 t/ha, 8.10 t/ha 2.85 t/ha, and grain in general - 5, 35 t/ha, which is 78.5-82.7% of the organo-mineral fertilizer system (table. 5). The amplitude range (max-min = Д) of the yield under the organic system of cultivation is shifted towards smaller absolute values of the interval and significantly narrowed compared to the organo-mineral fertilizer system. Similarly, the typical interval values change (L0.25-L0.75). Median yield values for the organic fertilizer system were lower than for the organo-mineral fertilizer system, but as in the latter case, their value tended to yield values for the upper typical value (L = 0.75), which indicates its growth in time measurement. The coefficient of variation of grain yield under the organic fertilizer system was lower, except for the yield of barley, compared with the organo-mineral fertilizer system: 7.95% vs. 10.5%.
Conclusions
A comparative assessment of the balance of phosphorus in 5-field grain row crop rotation when used as organic fertilizers by-products with saturation of crop rotation with legumes showed that the balance of phosphorus in the organo-mineral fertilizer system was positive (+17.7 kg/ha), while the balance of the organic fertilizer system was negative (-16.6 kg/ha), and the intensity and capacity of the phosphorus balance of the organic fertilizer system were 2.7 and 3.7 times lower compared to the organo-mineral fertilizer system.
In the organic system of fertilizer between productivity (yield of feed units and main products) and turnover rate and capacity of the balance of P2O5 revealed significant correlations: with productivity, the relationship was inverted (R = -0.65-0.69 ± 0.02; R2 = 0.42-0.48), and with the specified balance indicators straight (R = + 0.67-0.69 ± 0.02; R2 = 0.45-0.52). Compared with the organo-mineral fertilizer system, the correlations were somewhat weakened, and per unit yield k.o. and the main product is 1.78 and 2.11 times less than the increase in the capacity of the balance of P2O5.
Phosphorus balance capacity, as an estimated indicator of balance, proved to be a reliable criterion for assessing the interaction with the balance of N and CO2 balance for different fertilizer systems: a direct correlation was established at the average level: R = 0.53 ± 0.02; R2 = 0.23 for N and R = 0.90 ± 0.03; R2 =0. 86 - for CO2, and per unit growth of phosphorus balance capacity accounted for 7.62 kg N and 0.79 t of CO2, which is much higher compared to the organo-mineral fertilizer system and indicates the intensity of phosphorus balance in the organic fertilizer system.
Estimation of grain yield dynamics for 2011-2019 showed that regardless of the fertilization system, increasing yield trends were found in the cultivation of winter wheat and spring barley, and in the cultivation of corn yield trends were declining, but in the organic fertilization system trends were less declining, which made it possible to reach a yield level of about 7.0 t/ha, as in the organo-mineral fertilizer system. In general, grain yields in both organic and organo-mineral cultivation systems were declining, but in the organic fertilizer system the trend was 1.9 times smaller compared to the organo-mineral fertilizer system. The average grain yield under the organic fertilizer system was lower by 1.32 t/ha relative to the organic-mineral system, which was 6.67 t/ha.
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