Воздействие семян хлопчатника и сырых соевых бобов на изомеры линолевой кислоты и состав ненасыщенных жирных кислот в молоке овец

 

3>5 (P<0,02);4>5(P<0,04)

18:1щ7t

1>4 (P<0,001);1>5(P<0,002)

NS

18:1щ9C

1>2 (P<0,01);1>4(P<0,005)

2>4 (P<0,002)

18:1щ7C

1>2 (P<0,001);1>3(P<0,02)

1>2 (P<0,01);2>3(P<0,004)

 

2>5 (P<0,005);3>4(P<0,03)

3>4 (P<0,002);4>5(P<0,003)

18:2щ6t

1>2 (P<0,009);1>3(P<0,001)

1>5 (P<0,001);4>5(P<0,009)

 

2>4 (P<0,02);3>4(P<0,003)

 

18:2щ6C

1>3 (P<0,001);2>4(P<0,005)

1>3 (P<0,004);3>4(P<0,008)

 

3>4(P<0,01)

4>5 (P<0,003)

18:3щ3

1>2 (P<0,01);1>4(P<0,04)

1>5 (P<0,002);2>4(P<0,001)

 

2>3 (P<0,02);3>5(P<0,003)

2>5 (P<0,002);3>5(P<0,006)

CLA(c-9,t-11)

2>4 (P<0,01);2>5(P<0,02)

NS

20:5щ3

1>4 (P<0,003);1>5(P<0,003)

NS

22:6щ3

1>2 (P<0,003);1>3(P<0,003)

NS

* 1^st research week ***** 5^th research week

** 2^nd research week

*** 3^rd research week

**** 4^th research week

When full fat soybean (diet 2) was added into the diet (Table 5) almost the same dynamics was present as in diet 1,that is the increase of the treatment was accompanied by the decrease of 10:1, 14:1w9, acids. The level of 18:1w7t as in diet 1 increases during all five weeks of investigation. Regarding 16:1w7, it can be said that when the treatment is increased, the level of the acid decreases only during the first and fifth week of the experiment whereas during the rest time of the experiment the increase of the treatment was accompanied by the increase of the above-mentioned fatty acid. As to 17:1, its level compared with the control decreases when the treatment is increased during the first two weeks. Moreover, there are no differences observed in the second and third treatment. During the last three weeks of the experiment the level of 17:1 increases parallel with the treatment.

Regarding the CLA isomers(table 5), the results of the experiment indicate that in diet 1 (WCS) during the first two weeks of the investigation the level of predominant cis-9 trans-11 isomer in treatment 2 decreases as compared with the control . Whereas during the remaining three weeks of the experiment the treatment increase is accompanied by the increase of cis-9 trans-11 CLA isomer level. The same is true of diet 2.

In the level of the remaining CLA isomers trans-10, cis-12 and cis-9, cis-11 no changes were observed in neither of the diets.

Analyzing the influence of the diet on the PUFA level, in case of different treatments (Table 5) we can state that the supplementation of the diet with both WCS and full fat soybean depending on the treatment is not accompanied by any change in the level of 18:4w3; 20:3w6; 20:4w6; 22:4w6; 22:5w3; 22:5w6; 22:6w3 acids.

In case of diet 1 changes are evident in the level of 18:2w6t; 18:2w6c; 18:3w3, and 20:5w3.

Besides, basically with the increase of the treatment the content of the above mentioned fatty acids increases, and only the level of 18:3w6 decreases; whereas during the third week of the experiment no changes were observed. The quantity of 20:5w3 did not fluctuate during the second week of the experiment.

What refers to the full fat soybean supplementation, the quantity of 18:2w6t; 18:2w6c; 18:3w3 increases parallel with the increase of the treatment during all the weeks of the experiment. The changes in 20:5w3 are not significant, and during the third week of the experiment it levels, in fact, whereas the opposite takes place in the content of 18:3w6 acid-with the increase of the treatment the content slightly decreases.

Table 5. The content comparison of fatty acids in milk within 2,5 months of lactation period depending on the treatment.

UFA	Diet
g/100mL	WCS	SOYBEAN
of milk	Week	Treatment	Mean	Week	Treatment	Mean
10:1	1	1	0,028	1	1	0,027
		2	0,016		2	0,019
		3	0,009		3	0,016
	2	1	0,027	2	1	0,027
		2	0,017		2	0,020
		3	0,009		3	0,017
	3	1	0,026	3	1	0,036
		2	0,017		2	0,022
		3	0,009		3	0,025
	4	1	0,030	4	1	0,036
		2	0,016		2	0,024
		3	0,009		3	0,025
	5	1	0,028	5	1	0,031
		2	0,018		2	0,025
		3	0,011		3	0,020
14:1щ9	1	1	0,690	1	1	0,685
		2	0,540		2	0,577
		3	0,474		3	0,512
	2	1	0,657	2	1	0,626
		2	0,527		2	0,536
		3	0,411		3	0,489
	3	1	0,616	3	1	0,810
		2	0,533		2	0,591
		3	0,460		3	0,607
	4	1	0,808	4	1	0,849
		2	0,574		2	0,654
		3	0,499		3	0,703
	5	1	0,718	5	1	0,741
		2	0,572		2	0,625
		3	0,498		3	0,554

UFA	Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
16:1щ7	1	1	0,059	1	1	0,056
		2	0,037		2	0,045
		3	0,038		3	0,04
	2	1	0,054	2	1	0,059
		2	0,042		2	0,046
		3	0,035		3	0,048
	3	1	0,055	3	1	0,078
		2	0,043		2	0,054
		3	0,040		3	0,061
	4	1	0,078	4	1	0,092
		2	0,050		2	0,069
		3	0,050		3	0,077
	5	1	0,072	5	1	0,084
		2	0,053		2	0,068
		3	0,052		3	0,061
17:1щ7	1	1	0,014	1	1	0,027
		2	0,009		2	0,024
		3	0,008		3	0,025
	2	1	0,010	2	1	0,023
		2	0,008		2	0,021
		3	0,006		3	0,022
	3	1	0,010	3	1	0,028
		2	0,007		2	0,021
		3	0,007		3	0,023
	4	1	0,015	4	1	0,031
		2	0,009		2	0,030
		3	0,010		3	0,033
	5	1	0,015	5	1	0,027
		2	0,009		2	0,027
		3	0,010		3	0,026

	Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
18:1щ9t	1	1	0,026	1	1	0,024
		2	0,035		2	0,029
		3	0,056		3	0,035
	2	1	0,026	2	1	0,019
		2	0,033		2	0,024
		3	0,040		3	0,032
	3	1	0,021	3	1	0,024
		2	0,033		2	0,025
		3	0,049		3	0,029
	4	1	0,030	4	1	0,029
		2	0,042		2	0,032
		3	0,059		3	0,038
	5	1	0,026	5	1	0,023
		2	0,041		2	0,029
		3	0,043		3	0,038
18:1щ7t	1	1	0,027	1	1	0,023
		2	0,031		2	0,030
		3	0,049		3	0,030
	2	1	0,024	2	1	0,018
		2	0,033		2	0,025
		3	0,054		3	0,029
	3	1	0,024	3	1	0,024
		2	0,034		2	0,031
		3	0,056		3	0,032
	4	1	0,021	4	1	0,029
		2	0,040		2	0,034
		3	0,040		3	0,039
	5	1	0,026	5	1	0,029
		2	0,049		2	0,038
		3	0,052		3	0,038

UFA			Diet
	WCS		SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
18:1щ9c	1	1	1,017	1	1	0,989
		2	1,104		2	1,284
		3	1,205		3	1,478
	2	1	0,805	2	1	0,858
		2	1,020		2	1,130
		3	1,012		3	1,264
	3	1	0,781	3	1	1,139
		2	1,004		2	1,196
		3	1,203		3	1,479
	4	1	1,196	4	1	1,277
		2	1,309		2	1,563
		3	1,539		3	1,949
	5	1	0,856	5	1	0,986
		2	1,127		2	1,316
		3	1,697		3	1,516
18:1щ7c	1	1	0,026	1	1	0,025
		2	0,022		2	0,021
		3	0,031		3	0,022
	2	1	0,023	2	1	0,023
		2	0,018		2	0,017
		3	0,019		3	0,021
	3	1	0,022	3	1	0,029
		2	0,021		2	0,019
		3	0,022		3	0,022
	4	1	0,034	4	1	0,033
		2	0,021		2	0,023
		3	0,032		3	0,029
	5	1	0,033	5	1	0,027
		2	0,027		2	0,023
		3	0,030		3	0,023

UFA	Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
18:2щ6t	1	1	0,015	1	1	0,016
		2	0,034		2	0,027
		3	0,048		3	0,036
	2	1	0,015	2	1	0,012
		2	0,029		2	0,020
		3	0,035		3	0,027
	3	1	0,012	3	1	0,014
		2	0,026		2	0,019
		3	0,034		3	0,025
	4	1	0,017	4	1	0,018
		2	0,032		2	0,026
		3	0,042		3	0,034
	5	1	0,013	5	1	0,014
		2	0,031		2	0,023
		3	0,040		3	0,032
18:2щ6c	1	1	0,171	1	1	0,148
		2	0,174		2	0,163
		3	0,215		3	0,235
	2	1	0,116	2	1	0,110
		2	0,145		2	0,133
		3	0,157		3	0,194
	3	1	0,102	3	1	0,149
		2	0,139		2	0,140
		3	0,179		3	0,186
	4	1	0,141	4	1	0,154
		2	0,156		2	0,164
		3	0,231		3	0,234
	5	1	0,123	5	1	0,134
		2	0,155		2	0,142
		3	0,238		3	0,196

UFA	Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
18:3щ6	1	1	0,005	1	1	0,005
		2	0,004		2	0,003
		3	0,004		3	0,003
	2	1	0,004	2	1	0,004
		2	0,004		2	0,003
		3	0,003		3	0,003
	3	1	0,004	3	1	0,006
		2	0,004		2	0,003
		3	0,004		3	0,004
	4	1	0,006	4	1	0,008
		2	0,005		2	0,006
		3	0,004		3	0,007
	5	1	0,006	5	1	0,008
		2	0,006		2	0,006
		3	0,005		3	0,006
18:3щ3	1	1	0,030	1	1	0,023
		2	0,020		2	0,025
		3	0,019		3	0,034
	2	1	0,020	2	1	0,017
		2	0,017		2	0,021
		3	0,014		3	0,029
	3	1	0,015	3	1	0,021
		2	0,013		2	0,019
		3	0,012		3	0,024
	4	1	0,023	4	1	0,022
		2	0,016		2	0,026
		3	0,017		3	0,033
	5	1	0,021	5	1	0,021
		2	0,018		2	0,024
		3	0,023		3	0,030

UFA	Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
CLA(c-9, t-11)	1	1	0,047	1	1
		2	0,031		2	0,031
		3	0,043		3	0,043
	2	1	0,033	2	1
		2	0,024		2	0,024
		3	0,033		3	0,033
	3	1	0,026	3	1
		2	0,027		2	0,027
		3	0,059		3	0,059
	4	1	0,037	4	1
		2	0,036		2	0,036
		3	0,074		3	0,074
	5	1	0,044	5	1
		2	0,041		2	0,003
		3	0,057		3	0,057
CLA(t-10, c-12)	1	1	0,002	1	1	0,002
		2	0,002		2	0,002
		3	0,002		3	0,002
	2	1	0,002	2	1	0,002
		2	0,002		2	0,002
		3	0,001		3	0,001
	3	1	0,001	3	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
	4	1	0,001	4	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
	5	1	0,001	5	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001

	Week	Treatment	Mean	Week	Treatment	Mean
CLA c-9,c-11	1	1	0,001	1	1	0,002
		2	0,001		2	0,001
		3	0,001		3	0,001
	2	1	0,001	2	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
	3	1	0,001	3	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
	4	1	0,001	4	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
	5	1	0,001	5	1	0,001
		2	0,001		2	0,001
		3	0,001		3	0,001
18:4щ3	1	1	0,002	1	1	0,002
		2	0,002		2	0,002
		3	0,002		3	0,002
	2	1	0,002	2	1	0,002
		2	0,002		2	0,002
		3	0,002		3	0,002
	3	1	0,002	3	1	0,002
		2	0,002		2	0,002
		3	0,002		3	0,002
	4	1	0,002	4	1	0,002
		2	0,002		2	0,001
		3	0,002		3	0,001
	5	1	0,001	5	1	0,002
		2	0,001		2	0,002
		3	0,001		3	0,002

UFA			Diet
	WCS	SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
20:3щ6	1	1	0,001	1	1	0,002
		2	0,001		2	0,001
		3	0,002		3	0,001
	2	1	0,001	2	1	0,002
		2	0,001		2	0,001
		3	0,001		3	0,001
	3	1	0,001	3	1	0,002
		2	0,002		2	0,001
		3	0,002		3	0,001
	4	1	0,004	4	1	0,005
		2	0,004		2	0,006
		3	0,005		3	0,005
	5	1	0,004	5	1	0,006
		2	0,005		2	0,005
		3	0,006		3	0,006
20:4щ6	1	1	0,003	1	1	0,003
		2	0,003		2	0,003
		3	0,003		3	0,003
	2	1	0,002	2	1	0,002
		2	0,002		2	0,002
		3	0,003		3	0,002
	3	1	0,002	3	1	0,003
		2	0,002		2	0,002
		3	0,003		3	0,003
	4	1	0,004	4	1	0,005
		2	0,004		2	0,006
		3	0,005		3	0,005
	5	1	0,004	5	1	0,006
		2	0,005		2	0,005
		3	0,006		3	0,006

UFA			Diet
	WCS		SOYBEAN
	Week	Treatment	Mean	WEEK	Treatment	Mean
20:5щ3	1	1	0,005	1	1	0,005
		2	0,005		2	0,008
		3	0,007		3	0,007
	2	1	0,005	2	1	0,005
		2	0,005		2	0,006
		3	0,005		3	0,006
	3	1	0,004	3	1	0,006
		2	0,004		2	0,006
		3	0,006		3	0,006
	4	1	0,007	4	1	0,009
		2	0,007		2	0,011
		3	0,009		3	0,011
	5	1	0,007	5	1	0,008
		2	0,007		2	0,010
		3	0,008		3	0,010
22:4щ6	1	1	0,002	1	1	0,003
		2	0,002		2	0,002
		3	0,003		3	0,002
	2	1	0,002	2	1	0,003
		2	0,002		2	0,002
		3	0,002		3	0,002
	3	1	0,002	3	1	0,003
		2	0,003		2	0,003
		3	0,004		3	0,002
	4	1	0,004	4	1	0,006
		2	0,004		2	0,006
		3	0,005		3	0,007
	5	1	0,005	5	1	0,007
		2	0,005		2	0,006
		3	0,006		3	0,006

UFA			Diet
	WCS		SOYBEAN
	Week	Treatment	Mean	Week	Treatment	Mean
22:6щ3	1	1	0,009	1	1	0,007
		2	0,006		2	0,006
		3	0,006		3	0,007
	2	1	0,006	2	1	0,005
		2	0,004		2	0,005
		3	0,004		3	0,005
	3	1	0,004	3	1	0,006
		2	0,005		2	0,005
		3	0,005		3	0,005
	4	1	0,008	4	1	0,010
		2	0,006		2	0,009
		3	0,008		3	0,010
	5	1	0,008	5	1	0,008
		2	0,006		2	0,008
		3	0,007		3	0,009

Conclusion

The results of this study showed that the supplementation of dairy sheep diet with both WCS and full fat soybean significantly increased parallel with the treatment the level 18:1w9t and 18:1w9c MUFA. Accordingly, the content of PUFA in case of WCS supplementation the level of 18:2w6t and 18:2w6c fatty acids ahs resulted in the significant increase. As compared with the first diet, when the diet is supplemented with full fat soybean, a significant increase becomes evident in the level of 18:3w3 fatty acid. As to the rest of fatty acids, no changes have been observed.

Regarding the CLA isomers, it is only the level of the CLA predominant isomer cis-9. trans-11, that significantly increases when the WCS is added into the diet of ewes with the treatment of 2->3 , where no changes take place in the content of the other two isomers trans-10, cis-12 and cis-9, cis-11.

The diet within the week shows that the supplementation of the diet with WCS contributes to the increase of MUFA content during a five-week research period.

During the five-week research on the treatment within the diet, of all CLA isomers under study it was only the level of cis-9, trans-11 isomer that increased significantly when the diet was supplemented with WCS; whereas the level of the remaining CLA isomers almost did not change -neither in the first diet nor in the second.

Regarding the treatment within five weeks, during the first two weeks of the investigation carried out in diet 1, the level of cis-9, trans-11 in treatment 2 decreased as compared with the content; whereas during the remaining three weeks the treatment increase was accompanied by the increase of this isomer. In the level of trans-10, cis-12 and cis-9, cis-11 no changes were observed in neither of the diets.

Overall, for both diets it is most suitable to use the third treatment in order to increase the level of cis-9, trans-11 CLA isomer in milk.

A necessity might arise to continue the research which might require the use of other treatments of WCS and full fat soybean with the aim of further increasing cis-9, trans-11 CLA isomer level.

References

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